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Image Search Results
Journal: Experimental and Therapeutic Medicine
Article Title: Electric pulse current stimulation increases electrophysiological properties of I f current reconstructed in mHCN4-transfected canine mesenchymal stem cells
doi: 10.3892/etm.2016.3072
Figure Lengend Snippet: Characteristics of functional expression of I f in mouse hyperpolarization-activated cyclic nucleotide-gated channel 4 (m HCN4 )-transfected canine msenchymal stem cells (cMSCs). (A) I f current recorded in group A. (B) Effect of extracellular Cs + on If. (C) Effect of EPCS on HCN4 -transfected cMSCs. (D) Voltage protocol enables the mensuration of the (E) reversal potentials. (F) Representative tail currents used to construct the I f activation curves. Data are presented as the mean ± standard error of the mean. EPCS, electric pulse current stimulation.
Article Snippet:
Techniques: Functional Assay, Expressing, Transfection, Construct, Activation Assay
Journal: Experimental and Therapeutic Medicine
Article Title: Electric pulse current stimulation increases electrophysiological properties of I f current reconstructed in mHCN4-transfected canine mesenchymal stem cells
doi: 10.3892/etm.2016.3072
Figure Lengend Snippet: Transmission electron micrographs of gap junctions in adjacent cells. (A) Gap junctions in hyperpolarization-activated cyclic nucleotide-gated channel 4 (m HCN4 )-transfected canine mesenchymal stem cells (cMSCs) (group A). (B) Gap junctions were increased in m HCN4 -cMSCs induced by EPCS (group B). Arrows indicate the gap junctions.
Article Snippet:
Techniques: Transmission Assay, Transfection
Journal: Experimental and Therapeutic Medicine
Article Title: Electric pulse current stimulation increases electrophysiological properties of I f current reconstructed in mHCN4-transfected canine mesenchymal stem cells
doi: 10.3892/etm.2016.3072
Figure Lengend Snippet: HCN4 , Cx43 and Cx45 gene expression were examined using reverse transcription-quantitative polymerase chain reaction. Data are presented as the mean ± standard error. *P<0.05 vs. HCN4 (group A). HCN4 , hyperpolarization-activated cyclic nucleotide-gated channel 4; EPCS, electric pulse current stimulation; Cx, connexin.
Article Snippet:
Techniques: Gene Expression, Reverse Transcription, Real-time Polymerase Chain Reaction
Journal: Experimental and Therapeutic Medicine
Article Title: Electric pulse current stimulation increases electrophysiological properties of I f current reconstructed in mHCN4-transfected canine mesenchymal stem cells
doi: 10.3892/etm.2016.3072
Figure Lengend Snippet: HCN4 , Cx43 and Cx45 protein expression was detected using western blot analysis. HCN4 , hyperpolarization-activated cyclic nucleotide-gated channel 4; EPCS, electric pulse current stimulation; Cx, connexin.
Article Snippet:
Techniques: Expressing, Western Blot